Download Apoptosis Methods in Toxicology by Perpetua M. Muganda PDF

By Perpetua M. Muganda

ISBN-10: 1493935860

ISBN-13: 9781493935864

ISBN-10: 1493935887

ISBN-13: 9781493935888

This precise quantity offers a unmarried, priceless reference resource for ways that definitively determine and safely quantify apoptosis. The booklet starts off with universal equipment applied to realize and quantitate apoptosis, in addition to apoptosis signaling pathways in toxicological and different similar examine. It keeps with multi-parametric and phased apoptosis assays for detecting early and overdue apoptosis or distinguishing apoptosis from necrosis and autophagy. next chapters concentrate on fresh advances in genuine time and high-throughput assays that realize and quantitate apoptosis and apoptosis signaling pathways. ultimate chapters concentrate on fresh advancements in preclinical anticancer therapeutics focusing on apoptosis. Written for the Methods in Pharmacology and Toxicology sequence, chapters function step by step descriptions of the methodologies, in addition to specialist suggestions and implementation advice.

Vital and authoritative, Apoptosis tools in Toxicology serves amateur scientists in addition to specialists, using more than a few tools from universal laboratory gear to high-end pricey and automatic equipment in a position to appearing actual time apoptotic measurements.

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Shaker. 4. Microplate(s) suitable for cell culture and for use in luminometer. 5. 1 coverslips. 36 Akamu J. Ewunkem and Perpetua M. 3 Nuclear Morphology Fluorescent Dual Dye Staining Assay 1. Acridine orange, 10 mg/ml solution (Thermo Fisher Scientific Life Technologies). This is a cell permeable nucleic acid binding dye that stains the nucleus green. It is stored in a dark container at 4 °C, since it is light sensitive. It is also a mutagen; thus every precaution should be taken to protect personnel and environment from exposure.

Steps 1–5 are carried out under class 2 type A2 conditions to protect personnel as well as the product. 1. Determine the cell count for all control and toxicant exposed samples that constitute the experiment. Perform calculations needed to deliver 1 × 104 cells in 100 μl of media for each sample within the experiment in designated wells within a 96-well plate (see Note 5). 2. Remove from the freezer and equilibrate the Caspase-3/7-Glo® substrate and buffer to room temperature; place in a room temperature shallow water bath for approximately 30 min.

118. 119. 120. to a novel small-molecule detector of apoptosis. Cell Res 19:625–637 Hoglund J, Shirvan A, Antoni G, Gustavsson SA, Langstrom B, Ringheim A et al (2011) 18F-ML-10, a PET tracer for apoptosis: first human study. J Nucl Med 52:720–725 Cazzaniga M, Decensi A, Pruneri G, Puntoni M, Bottiglieri L, Varricchio C et al (2013) The effect of metformin on apoptosis in a breast cancer presurgical trial. Br J Cancer 109:2792–2797 Dai G, Tong Y, Chen X, Ren Z, Ying X, Yang F et al (2015) Myricanol induces apoptotic cell death and anti-tumor activity in nonsmall cell lung carcinoma in vivo.

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