Download Apoptosis Methods in Pharmacology and Toxicology: Approaches by Myrtle A. Davis PDF

By Myrtle A. Davis

ISBN-10: 0896038904

ISBN-13: 9780896038905

Univ. of Maryland, Baltimore. Discusses laser scanning cytometry for the research of apoptosis, tissue-based equipment of quantification for reviews utilizing animal versions, and practical assays and ELISA recommendations. DNLM: Apoptosis--physiology.

Show description

Read or Download Apoptosis Methods in Pharmacology and Toxicology: Approaches to Measurement and Quantification PDF

Best toxicology books

Pesticides - A Medical Dictionary, Bibliography, and Annotated Research Guide to Internet References

It is a 3-in-1 reference publication. It offers a whole scientific dictionary overlaying enormous quantities of phrases and expressions on the subject of insecticides. It additionally provides huge lists of bibliographic citations. eventually, it presents details to clients on tips on how to replace their wisdom utilizing a number of web assets.

Toxic Legacy: Synthetic Toxins in the Food, Water and Air of American Cities

Any expert exam of present or capability new pollutants in a inhabitants needs to account for these already current from earlier difficulties and ordinary stipulations. poisonous Legacy offers wide info at the incidence of chemical dangers and their strength risks in mixtures within the meals, water and air in towns round the usa.

Food toxicology

New info regularly point out that antioxidants may well give a contribution to savings in melanoma hazards and that power intake of low degrees of chemical cancer agents in our nutrition may well give a contribution to an elevated probability of constructing particular sorts of cancers. examine additionally exhibits that during the USA this day, the major explanations of demise are melanoma and center sickness.

Guardians of Science: Fairness and Reliability of Peer Review

An research of 1 of the main debatable problems with sleek medical tradition: peer overview has been the subject material of many articles in newspapers and basic curiosity medical and information magazines. This booklet considers the need of getting a competent approach to isolating the wheat from the chaff in technological know-how, within the gentle of the massive variety of fresh circumstances of fraud in technological know-how.

Additional resources for Apoptosis Methods in Pharmacology and Toxicology: Approaches to Measurement and Quantification

Example text

Applications for studies of apoptosis that descend from these unique features of LSC are presented further in this chapter. 3. ATTACHMENT OF CELLS TO MICROSCOPE SLIDES FOR ANALYSIS BY LSC Many assays of apoptosis by LSC are performed on fixed cells. For these assays the cells are attached to microscope slides by standard methods that include smear films, tissue sections, “touch” preparations from freshly transected tissues, or cytocentrifuging cell suspensions. Cytocentrifugation (see below) is often preferred over “touch” or smear preparations because it flattens cells on the slides so that their geometry is favorable, and therefore more morphological details can be revealed.

Science 283(5407), 1488–1493. 20. Haugland, R. (1999) Handbook of Fluorescent Probes and Research Chemicals. , Eugene, OR. 21. Poot, M. and Pierce, R. H. (1999) Detection of changes in mitochondrial function during apoptosis by simultaneous staining with multiple fluorescent dyes and correlated multiparameter flow cytometry. Cytometry 35(4): 311–317. 22. Lemasters, J. , Bradham, C. , Brenner, D. , Cascio, W. , Trost, L. , et al. (1999) Mitochondrial dysfunction in the pathogenesis of necrotic and apoptotic cell death.

Elmore, S. , et al. (1998) The mitochondrial permeability transition in cell death: a common mechanism in necrosis, apoptosis and autophagy. Biochim. Biophys. Acta 1366(1-2), 177–196. , and Tsujimoto, Y. (1999) Bcl-2 family proteins regulate the release of apoptogenic cytochrome c by the mitochondrial channel VDAC [see comments]. Nature 399(6735), 483–487. Finucane, D. , Waterhouse, N. , Cotter, T. , and Green, D. R. (1999) Bax-induced caspase activation and apoptosis via cytochrome c release from mitochondria is inhibitable by Bcl-xL.

Download PDF sample

Rated 4.89 of 5 – based on 23 votes